Multiplexed imaging and automated signal quantification in formalin-fixed paraffin-embedded tissues by ChipCytometry
Jarosch, S. K., J.; Sarker, R.S.J.; Steiger, K.; Janssen, K.-P.; Christians, A.; Hennig, C.; Holler, E.; D'Ippolito; E., Busch, D.H. (2021). Cell Reports Methods 1, art. no. 100104. doi: 10.1016/j.crmeth.2021.100104
Understanding of tissue biology requires in situ analyses of cell phenotypes. Jarosch et al. describe the application of the multiplexed imaging method ChipCytometry to formalin-fixed and paraffin-embedded tissue sections. The high staining quality allowed them to develop a pipeline for automated signal quantifications facilitating reliable and reproducible analyses of high-parameter imaging.