Modification of radiosensitivity by Curcumin in human pancreatic cancer cell lines
Schwarz, K., Dobiasch, S., Nguyen, L., Schilling, D., and Combs, S. E.
(2020). Sci Rep 10, 3815
Abstract: Pancreatic cancer
is one of the most aggressive malignancies and is characterized by a low 5-year survival rate, a broad genetic diversity and a high resistance to conventional therapies. As a result, novel therapeutic agents to improve the current situation are needed urgently. Curcumin
, a polyphenolic colorant derived from Curcuma longa root, showed pleiotropic influences on cellular pathways in vitro and amongst others anti-cancer
properties including sensitization of tumor cells to chemo- and radiation-therapy. In this study, we evaluated the impact of Curcumin
on the radiosensitivity
of the established human pancreatic cancer cell lines
Panc-1 and MiaPaCa-2 in vitro. In contrast to MiaPaCa-2 cells, we found a significant radiosensitization by Curcumin
in the more radioresistant Panc-1 cells, possibly caused by cell
cycle arrest in the most radiation-sensitive G2/M-phase at the time of irradiation. Furthermore, a significant enhancement of radiation-induced apoptosis, DNA-double-strand breaks and G2/M-arrest after curcumin
treatment was observed in both cell lines
. These in vitro findings suggest that especially patients with more radioresistant tumors could benefit from a radiation-concomitant, phytotherapeutic therapy with Curcumin.